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Indigo carmine

Name: Indigo carmine
Brand: TargetMol
SKU: T19755
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T19755Cas No. 860-22-0

Alias NSC-8646, NSC8646, NSC 8646, Indigotindisulfonate sodium, Brilliant Indigo

Indigo carmine (NSC-8646) is an indolesulfonic acid. It is used as a dye in renal function testing for the detection of nitrates and chlorates.

Indigo carmine

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Purity: 99.85%

Catalog No. T19755Alias NSC-8646, NSC8646, NSC 8646, Indigotindisulfonate sodium, Brilliant IndigoCas No. 860-22-0

Indigo carmine (NSC-8646) is an indolesulfonic acid. It is used as a dye in renal function testing for the detection of nitrates and chlorates.

Pack Size	Price	USA Warehouse	Global Warehouse
200 mg	$30	In Stock	In Stock
500 mg	$41	In Stock	In Stock
1 g	$56	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.85%

Color:Blue to Purple

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	Indigo carmine (NSC-8646) is an indolesulfonic acid. It is used as a dye in renal function testing for the detection of nitrates and chlorates.
Animal Research	Instructions Operation steps 1. Dissolve Indigo Carmine in saline or PBS. 2. Inject intravenously or locally into the target area. Common dose: 0.25-3 mg/kg (adjusted according to animal species and experimental requirements).
Synonyms	NSC-8646, NSC8646, NSC 8646, Indigotindisulfonate sodium, Brilliant Indigo

Chemical Properties

Molecular Weight	466.35
Formula	C₁₆H₈N₂Na₂O₈S₂
Cas No.	860-22-0
Smiles	O=S(C1=CC2=C(N/C(C2=O)=C3NC4=C(C=C(S(=O)([O-])=O)C=C4)C\3=O)C=C1)([O-])=O.[Na+].[Na+]
Relative Density.	0.666 g/cm3. Temperature:29.05 °C.;0.714 g/cm3. Temperature:29.05 °C.

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 18 mg/mL (38.6 mM), Sonication is recommended.

DMSO: Soluble

Solution Preparation Table

H2O

	1mg	5mg	10mg	50mg
1 mM	2.1443 mL	10.7216 mL	21.4431 mL	107.2156 mL
5 mM	0.4289 mL	2.1443 mL	4.2886 mL	21.4431 mL
10 mM	0.2144 mL	1.0722 mL	2.1443 mL	10.7216 mL
20 mM	0.1072 mL	0.5361 mL	1.0722 mL	5.3608 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc